Title |
Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae
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Published in |
BMC Microbiology, October 2013
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DOI | 10.1186/1471-2180-13-229 |
Pubmed ID | |
Authors |
Lauren C Liddell, Glenn M Manthey, Shannon N Owens, Becky XH Fu, Adam M Bailis |
Abstract |
In Saccharomyces cerevisiae, Rad59 is required for multiple homologous recombination mechanisms and viability in DNA replication-defective rad27 mutant cells. Recently, four rad59 missense alleles were found to have distinct effects on homologous recombination that are consistent with separation-of-function mutations. The rad59-K166A allele alters an amino acid in a conserved α-helical domain, and, like the rad59 null allele diminishes association of Rad52 with double-strand breaks. The rad59-K174A and rad59-F180A alleles alter amino acids in the same domain and have genetically similar effects on homologous recombination. The rad59-Y92A allele alters a conserved amino acid in a separate domain, has genetically distinct effects on homologous recombination, and does not diminish association of Rad52 with double-strand breaks. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 31% |
Lecturer > Senior Lecturer | 1 | 8% |
Professor | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Agricultural and Biological Sciences | 3 | 23% |
Immunology and Microbiology | 1 | 8% |
Unknown | 6 | 46% |