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Mendeley readers
Chapter title |
Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and De Novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labeled Docosanoic Acid
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Chapter number | 5 |
Book title |
Peroxisomes
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Published in |
Methods in molecular biology, April 2017
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DOI | 10.1007/978-1-4939-6937-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Malu-Clair van de Beek, Inge M. E. Dijkstra, Stephan Kemp |
Editors |
Michael Schrader |
Abstract |
Peroxisomes are present in virtually every eukaryotic cell type with the exception of the mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA; ≥22 carbon atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled docosanoic acid (D3-C22:0). |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 26% |
Researcher | 4 | 17% |
Professor | 3 | 13% |
Student > Bachelor | 3 | 13% |
Professor > Associate Professor | 1 | 4% |
Other | 0 | 0% |
Unknown | 6 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 35% |
Neuroscience | 3 | 13% |
Medicine and Dentistry | 3 | 13% |
Agricultural and Biological Sciences | 2 | 9% |
Business, Management and Accounting | 1 | 4% |
Other | 0 | 0% |
Unknown | 6 | 26% |