Chapter title |
Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3′-diaminobenzidine
|
---|---|
Chapter number | 10 |
Book title |
Peroxisomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
H. Dariush Fahimi |
Editors |
Michael Schrader |
Abstract |
Peroxisomes are ubiquitous dynamic and multifunctional organelles that contribute to numerous anabolic and catabolic pathways, being essential for human health and development. Their best known functions include the oxidation of fatty acids and metabolism of hydrogen peroxide with catalase as a marker enzyme. Indeed, historically, it was the cytochemical staining of catalase in many different cells and tissues that revealed the ubiquitous presence of peroxisomes in almost all animal and plant cells. In this chapter, the method for cytochemical staining of catalase with the alkaline 3, 3'-diaminobenzidine (DAB) is described. Since aldehyde fixation is a prerequisite for staining of catalase with DAB, a method for perfusion fixation of rat liver with glutaraldehyde is presented prior to the cytochemical staining method and the subsequent tissue processing for light and electron microscopy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 2 | 20% |
Student > Ph. D. Student | 1 | 10% |
Researcher | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Unknown | 5 | 50% |
Readers by discipline | Count | As % |
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Chemistry | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |
Unknown | 5 | 50% |