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Chapter title |
In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes
|
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Chapter number | 19 |
Book title |
Peroxisomes
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Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Yuqiong Liu, Masanori Honsho, Yukio Fujiki |
Editors |
Michael Schrader |
Abstract |
The Pex19p- and Pex3p-dependent direct import of peroxisomal membrane proteins (PMPs), termed the class I pathway, can be reconstituted in vitro by incubating cell-free synthesized PMPs with highly purified peroxisomes at 26 °C for 1 h. This method ensures that the proteins targeted to peroxisomes are imported directly without involvement of other organelles. |