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The expanding universe of transposon technologies for gene and cell engineering

Overview of attention for article published in Mobile DNA, January 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (61st percentile)

Mentioned by

twitter
3 tweeters
patent
2 patents

Readers on

mendeley
208 Mendeley
citeulike
2 CiteULike
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Title
The expanding universe of transposon technologies for gene and cell engineering
Published in
Mobile DNA, January 2010
DOI 10.1186/1759-8753-1-25
Pubmed ID
Authors

Zoltán Ivics, Zsuzsanna Izsvák

Abstract

Transposable elements can be viewed as natural DNA transfer vehicles that, similar to integrating viruses, are capable of efficient genomic insertion. The mobility of class II transposable elements (DNA transposons) can be controlled by conditionally providing the transposase component of the transposition reaction. Thus, a DNA of interest (be it a fluorescent marker, a small hairpin (sh)RNA expression cassette, a mutagenic gene trap or a therapeutic gene construct) cloned between the inverted repeat sequences of a transposon-based vector can be used for stable genomic insertion in a regulated and highly efficient manner. This methodological paradigm opened up a number of avenues for genome manipulations in vertebrates, including transgenesis for the generation of transgenic cells in tissue culture, the production of germline transgenic animals for basic and applied research, forward genetic screens for functional gene annotation in model species, and therapy of genetic disorders in humans. Sleeping Beauty (SB) was the first transposon shown to be capable of gene transfer in vertebrate cells, and recent results confirm that SB supports a full spectrum of genetic engineering including transgenesis, insertional mutagenesis, and therapeutic somatic gene transfer both ex vivo and in vivo. The first clinical application of the SB system will help to validate both the safety and efficacy of this approach. In this review, we describe the major transposon systems currently available (with special emphasis on SB), discuss the various parameters and considerations pertinent to their experimental use, and highlight the state of the art in transposon technology in diverse genetic applications.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 208 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
United Kingdom 2 <1%
Germany 2 <1%
Brazil 2 <1%
Peru 1 <1%
Czechia 1 <1%
Netherlands 1 <1%
Italy 1 <1%
Mexico 1 <1%
Other 5 2%
Unknown 189 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 46 22%
Student > Ph. D. Student 46 22%
Student > Bachelor 32 15%
Student > Master 25 12%
Student > Postgraduate 15 7%
Other 33 16%
Unknown 11 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 125 60%
Biochemistry, Genetics and Molecular Biology 46 22%
Medicine and Dentistry 9 4%
Immunology and Microbiology 3 1%
Nursing and Health Professions 2 <1%
Other 7 3%
Unknown 16 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2019.
All research outputs
#2,503,227
of 15,225,242 outputs
Outputs from Mobile DNA
#72
of 257 outputs
Outputs of similar age
#42,197
of 262,633 outputs
Outputs of similar age from Mobile DNA
#5
of 13 outputs
Altmetric has tracked 15,225,242 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 257 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,633 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.