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Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

Overview of attention for article published in AMB Express, May 2017
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

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2 tweeters
patent
1 patent
facebook
1 Facebook page

Citations

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11 Dimensions

Readers on

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124 Mendeley
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Title
Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli
Published in
AMB Express, May 2017
DOI 10.1186/s13568-017-0394-1
Pubmed ID
Authors

SooJin Han, Shushil Machhi, Mark Berge, Guoling Xi, Thomas Linke, Ronald Schoner

Abstract

Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an important role in targeting proteins for translocation across the cytoplasmic membrane to the periplasmic space and release into culture medium during the secretion process. Alpha toxinH35L (ATH35L) was selected as an antigen for vaccine development against Staphylococcus aureus infections. It was successfully secreted into culture medium of E. coli by using bacterial signal peptides linked to the N-terminus of the protein. In order to improve the level of secreted ATH35L, we designed a series of novel signal peptides by swapping individual domains of modifying dsbA and pelB signal peptides and tested them in a fed-batch fermentation process. The data showed that some of the modified signal peptides improved the secretion efficiency of ATH35L compared with E. coli signal peptides from dsbA, pelB and phoA proteins. Indeed, one of the novel signal peptides improved the yield of secreted ATH35L by 3.5-fold in a fed-batch fermentation process and at the same time maintained processing at the expected site for signal peptide cleavage. Potentially, these new novel signal peptides can be used to improve the secretion efficiency of other heterologous proteins in E. coli. Furthermore, analysis of the synthetic signal peptide amino acid sequences provides some insight into the sequence features within the signal peptide that influence secretion efficiency.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 124 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 20%
Researcher 20 16%
Student > Bachelor 18 15%
Student > Master 9 7%
Student > Postgraduate 6 5%
Other 10 8%
Unknown 36 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 39 31%
Agricultural and Biological Sciences 20 16%
Immunology and Microbiology 5 4%
Engineering 5 4%
Pharmacology, Toxicology and Pharmaceutical Science 4 3%
Other 15 12%
Unknown 36 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2020.
All research outputs
#5,015,827
of 17,764,497 outputs
Outputs from AMB Express
#101
of 1,082 outputs
Outputs of similar age
#89,009
of 274,333 outputs
Outputs of similar age from AMB Express
#3
of 19 outputs
Altmetric has tracked 17,764,497 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 1,082 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,333 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.