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The Renin-Angiotensin-Aldosterone System

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Cover of 'The Renin-Angiotensin-Aldosterone System'

Table of Contents

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    Book Overview
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    Chapter 1 A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques
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    Chapter 2 A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
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    Chapter 3 Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) Using Immunoblotting Approaches
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    Chapter 4 Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells
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    Chapter 5 Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta
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    Chapter 6 Measuring Blood Pressure Using a Noninvasive Tail Cuff Method in Mice
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    Chapter 7 Blood Pressure Monitoring Using Radio Telemetry Method in Mice
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    Chapter 8 Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
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    Chapter 9 Assessment of the Renin–Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria
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    Chapter 10 Comprehensive Assessments of Energy Balance in Mice
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    Chapter 11 In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis
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    Chapter 12 A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt
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    Chapter 13 Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
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    Chapter 14 A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection
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    Chapter 15 Analysis of Angiotensin Metabolism in the Kidney Using Mass Spectrometry
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    Chapter 16 Erratum to: Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
Attention for Chapter 2: A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
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Chapter title
A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
Chapter number 2
Book title
The Renin-Angiotensin-Aldosterone System
Published in
Methods in molecular biology, May 2017
DOI 10.1007/978-1-4939-7030-8_2
Pubmed ID
Book ISBNs
978-1-4939-7028-5, 978-1-4939-7030-8
Authors

Congqing Wu, Alan Daugherty, Hong Lu

Editors

Sean E. Thatcher

Abstract

There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

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Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 100%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 100%