Chapter title |
Analysis of Angiotensin Metabolism in the Kidney Using Mass Spectrometry
|
---|---|
Chapter number | 15 |
Book title |
The Renin-Angiotensin-Aldosterone System
|
Published in |
Methods in molecular biology, May 2017
|
DOI | 10.1007/978-1-4939-7030-8_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7028-5, 978-1-4939-7030-8
|
Authors |
Nadja Grobe, Khalid M. Elased, Grobe, Nadja, Elased, Khalid M. |
Editors |
Sean E. Thatcher |
Abstract |
The renin angiotensin system (RAS) is a highly complex enzymatic system consisting of multiple peptide hormones, enzymes, and receptors. A thorough characterization of angiotensin peptide metabolism is crucial for understanding pathological states associated with an imbalanced RAS. Here, we describe two matrix-assisted laser desorption/ionization (MALDI) mass spectrometric (MS) approaches for the assessment of in vitro and in situ RAS enzymatic activities in the kidney using the natural angiotensin peptide substrates. These MS techniques demonstrate high specificity and are superior over conventional spectrophotometric or colorimetric assays since multiple proteolytic cleavage sites can be detected, thus unraveling the complexity of the RAS. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 38% |
Professor | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 25% |
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Agricultural and Biological Sciences | 1 | 13% |
Computer Science | 1 | 13% |
Unknown | 3 | 38% |