Title |
Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis
|
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Published in |
BMC Veterinary Research, January 2014
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DOI | 10.1186/1746-6148-10-1 |
Pubmed ID | |
Authors |
Kathrin FA Langner, Alexa E Joetzke, Verena Nerschbach, Nina Eberle, Hans-Joachim Schuberth, Mirja Koy, Ingo Nolte, Daniela Betz |
Abstract |
Molecular techniques that detect canine lymphoma cells by their clonal antigen receptor gene rearrangement play an increasing role for diagnosis as well as for monitoring minimal residual disease during and after cytostatic therapy. However, the methods currently available are time-consuming and/or cost-intensive thus impeding the use in clinical routine. The aim of the present study was to develop and evaluate a real-time polymerase chain reaction (PCR) with subsequent melting curve analysis (MCA) for the detection of clonally rearranged antigen receptor genes in dogs with B and T cell lymphoma on non formalin-fixed and paraffin-embedded lymph node samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 2% |
United States | 1 | 2% |
Unknown | 64 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 8 | 12% |
Researcher | 8 | 12% |
Student > Postgraduate | 8 | 12% |
Student > Bachelor | 7 | 11% |
Student > Ph. D. Student | 5 | 8% |
Other | 18 | 27% |
Unknown | 12 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 22 | 33% |
Medicine and Dentistry | 11 | 17% |
Agricultural and Biological Sciences | 9 | 14% |
Biochemistry, Genetics and Molecular Biology | 6 | 9% |
Unspecified | 2 | 3% |
Other | 2 | 3% |
Unknown | 14 | 21% |