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Evaluation of a novel approach to circulating tumor cell isolation for cancer gene panel analysis in patients with breast cancer

Overview of attention for article published in Oncology Letters, March 2017
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Title
Evaluation of a novel approach to circulating tumor cell isolation for cancer gene panel analysis in patients with breast cancer
Published in
Oncology Letters, March 2017
DOI 10.3892/ol.2017.5807
Pubmed ID
Authors

Soo Jeong Lee, Cham Han Lee, Sung Ho Choi, Sei Hyun Ahn, Byung Ho Son, Jong Won Lee, Jong Han Yu, Nak-Jung Kwon, Woo Chung Lee, Kap-Seok Yang, Dong Hyoung Lee, Du Yeol Han, Mi So Choi, Pyeong-Soo Park, Hyun Kyung Lee, Myoung Shin Kim, Jinseon Lee, Byung Hee Jeon

Abstract

Liquid biopsy isolation of circulating tumor cells (CTCs) allows the genomic analysis of CTCs, which is useful in the determination of personalized cancer therapy. In the present study, CTCs from patients with breast cancer were enriched and successfully analyzed using cancer gene panel analysis. Blood samples from 11 patients with breast cancer were collected and CTCs enriched for using size-based filtration. The enriched CTCs were analyzed using immunofluorescence staining with antibodies directed against epithelial cell adhesion molecule (EpCAM) and cluster of differentiation 45. The genomic DNA of CTCs was extracted, amplified and 50 genes screened for mutations using the Ion AmpliSeq™ Cancer Hotspot Panel v2. EpCAM staining detected CTCs in 10/11 patients and the average CTC count was 3.9 in 5 ml blood. The average purity of enriched CTCs was 14.2±29.4% and the average amount of amplified DNA was 28.6±11.9 µg. Catalogue Of Somatic Mutations In Cancer mutations were detected in the CTCs and included IDH2, TP53, NRAS, IDH1, PDGFRA, HRAS, STK11, EGFR, PTEN, MLH1, PIK3CA, CDKN2A, KIT and SMARCB1. In conclusion, a novel size-based filtration approach for the isolation of CTCs was evaluated and successfully applied for the genomic analysis of CTCs from patients with breast cancer.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 2 17%
Professor > Associate Professor 2 17%
Researcher 2 17%
Student > Ph. D. Student 2 17%
Student > Postgraduate 1 8%
Other 3 25%
Readers by discipline Count As %
Unspecified 3 25%
Biochemistry, Genetics and Molecular Biology 3 25%
Medicine and Dentistry 3 25%
Agricultural and Biological Sciences 2 17%
Nursing and Health Professions 1 8%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2017.
All research outputs
#9,327,317
of 10,604,514 outputs
Outputs from Oncology Letters
#885
of 1,705 outputs
Outputs of similar age
#219,061
of 263,284 outputs
Outputs of similar age from Oncology Letters
#54
of 152 outputs
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So far Altmetric has tracked 1,705 research outputs from this source. They receive a mean Attention Score of 1.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 152 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.