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Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies

Overview of attention for article published in BMC Microbiology, May 2017
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Title
Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies
Published in
BMC Microbiology, May 2017
DOI 10.1186/s12866-017-1025-3
Pubmed ID
Authors

Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li

Abstract

Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively. The mAb isotypes were 11 (50%) IgG2a, 5 (23%) IgG1 and 6 (27%) IgM. On the basis of epitope recognition and reactivity levels, 8 mAbs including 3 IgM and 5 IgG clones were considered as highly reactive and potentially diagnostic antibodies. Among these mAbs, 7A3 (IgG1), 5B1 (IgG2a), 2C1 (IgG2a) and 5B3 (IgG2a) reacted with differently conserved linear epitopes of B. melitensis, B. ovis, B. suis and B. canis strains, while 5H3 (IgG2a) highly reacted with a conformational epitope of Omp31 when tested with several immunoassays. These potent monoclonal antibodies can be used for identifying Omp31 antigens or detecting B. melitensis and other Brucella species beyond B. abortus in vitro or in vivo.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 24%
Researcher 2 12%
Student > Master 2 12%
Student > Postgraduate 1 6%
Professor 1 6%
Other 3 18%
Unknown 4 24%
Readers by discipline Count As %
Immunology and Microbiology 3 18%
Agricultural and Biological Sciences 2 12%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Nursing and Health Professions 1 6%
Psychology 1 6%
Other 1 6%
Unknown 8 47%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2017.
All research outputs
#9,327,788
of 10,604,514 outputs
Outputs from BMC Microbiology
#1,312
of 1,583 outputs
Outputs of similar age
#219,069
of 263,283 outputs
Outputs of similar age from BMC Microbiology
#40
of 50 outputs
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