Title |
A family of GFP-like proteins with different spectral properties in lancelet Branchiostoma floridae
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Published in |
Biology Direct, July 2008
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DOI | 10.1186/1745-6150-3-28 |
Pubmed ID | |
Authors |
Diana Baumann, Malcolm Cook, Limei Ma, Arcady Mushegian, Erik Sanders, Joel Schwartz, C Ron Yu |
Abstract |
Members of the green fluorescent protein (GFP) family share sequence similarity and the 11-stranded beta-barrel fold. Fluorescence or bright coloration, observed in many members of this family, is enabled by the intrinsic properties of the polypeptide chain itself, without the requirement for cofactors. Amino acid sequence of fluorescent proteins can be altered by genetic engineering to produce variants with different spectral properties, suitable for direct visualization of molecular and cellular processes. Naturally occurring GFP-like proteins include fluorescent proteins from cnidarians of the Hydrozoa and Anthozoa classes, and from copepods of the Pontellidae family, as well as non-fluorescent proteins from Anthozoa. Recently, an mRNA encoding a fluorescent GFP-like protein AmphiGFP, related to GFP from Pontellidae, has been isolated from the lancelet Branchiostoma floridae, a cephalochordate (Deheyn et al., Biol Bull, 2007 213:95). |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 1% |
Sweden | 1 | 1% |
United Kingdom | 1 | 1% |
Belgium | 1 | 1% |
Spain | 1 | 1% |
Poland | 1 | 1% |
Unknown | 63 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 19 | 28% |
Researcher | 18 | 26% |
Student > Master | 8 | 12% |
Student > Bachelor | 7 | 10% |
Professor | 3 | 4% |
Other | 6 | 9% |
Unknown | 8 | 12% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 27 | 39% |
Biochemistry, Genetics and Molecular Biology | 10 | 14% |
Chemistry | 7 | 10% |
Earth and Planetary Sciences | 3 | 4% |
Physics and Astronomy | 3 | 4% |
Other | 11 | 16% |
Unknown | 8 | 12% |