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Mendeley readers
| Chapter title |
Expression of Plant Receptor Kinases in Yeast
|
|---|---|
| Chapter number | 2 |
| Book title |
Plant Receptor Kinases
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7063-6_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7062-9, 978-1-4939-7063-6
|
| Authors |
María Laura Barberini, Jorge P. Muschietti, Barberini, María Laura, Muschietti, Jorge P. |
| Abstract |
The budding yeast Saccharomyces cerevisiae is a useful system to express recombinant proteins and analyze protein-protein interaction. Membrane-spanning proteins like plant receptor kinases find their way to the plasma membrane when expressed in yeast and seem to retain their structure and function. Here, we describe a general yeast DNA transformation procedure based on lithium acetate, salmon sperm DNA, and polyethylene glycol used to express recombinant proteins. Yeast cells expressing plant receptor kinases can be used for in vivo and in vitro studies of receptor function. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 25% |
| Researcher | 2 | 25% |
| Student > Ph. D. Student | 1 | 13% |
| Unknown | 3 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 25% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Medicine and Dentistry | 1 | 13% |
| Unknown | 4 | 50% |