Title |
Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium
|
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Published in |
Journal of Biological Engineering, January 2014
|
DOI | 10.1186/1754-1611-8-4 |
Pubmed ID | |
Authors |
Daniel Camsund, Thorsten Heidorn, Peter Lindblad |
Abstract |
Cyanobacteria are solar-powered prokaryotes useful for sustainable production of valuable molecules, but orthogonal and regulated promoters are lacking. The Lac repressor (LacI) from Escherichia coli is a well-studied transcription factor that is orthogonal to cyanobacteria and represses transcription by binding a primary lac operator (lacO), blocking RNA-polymerase. Repression can be enhanced through DNA-looping, when a LacI-tetramer binds two spatially separated lacO and loops the DNA. Ptrc is a commonly used LacI-repressed promoter that is inefficiently repressed in the cyanobacterium Synechocystis PCC 6803. Ptrc2O, a version of Ptrc with two lacO, is more efficiently repressed, indicating DNA-looping. To investigate the inefficient repression of Ptrc and cyanobacterial DNA-looping, we designed a Ptrc-derived promoter library consisting of single lacO promoters, including a version of Ptrc with a stronger lacO (Ptrc1O-proximal), and dual lacO promoters with varying inter-lacO distances (the Ptrc2O-library). |
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United Kingdom | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
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Members of the public | 6 | 86% |
Practitioners (doctors, other healthcare professionals) | 1 | 14% |
Mendeley readers
Geographical breakdown
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United Kingdom | 2 | 1% |
Netherlands | 1 | <1% |
France | 1 | <1% |
China | 1 | <1% |
Iran, Islamic Republic of | 1 | <1% |
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Researcher | 36 | 19% |
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Student > Doctoral Student | 11 | 6% |
Other | 17 | 9% |
Unknown | 29 | 15% |
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Chemical Engineering | 9 | 5% |
Immunology and Microbiology | 3 | 2% |
Other | 8 | 4% |
Unknown | 30 | 16% |