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Ebolaviruses

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Cover of 'Ebolaviruses'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Marburg- and Ebolaviruses: A Look Back and Lessons for the Future
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    Chapter 2 Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Development and Application of New Technologies
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    Chapter 3 Ebolavirus: An Overview of Molecular and Clinical Pathogenesis
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    Chapter 4 Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms
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    Chapter 5 Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
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    Chapter 6 Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
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    Chapter 7 Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
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    Chapter 8 Modeling Ebolavirus Budding with Virus Like Particles
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    Chapter 9 Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays
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    Chapter 10 Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins
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    Chapter 11 Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA
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    Chapter 12 A Semi-automated High-Throughput Microtitration Assay for Filoviruses
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    Chapter 13 Generation of Recombinant Ebola Viruses Using Reverse Genetics
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    Chapter 14 Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
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    Chapter 15 Live-Cell Imaging of Filoviruses
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    Chapter 16 Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds
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    Chapter 17 Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence
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    Chapter 18 Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells
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    Chapter 19 Electron Microscopy of Ebola Virus-Infected Cells
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    Chapter 20 Validating the Inactivation Effectiveness of Chemicals on Ebola Virus
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    Chapter 21 Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)
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    Chapter 22 Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection
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    Chapter 23 Evaluation of Ebola Virus Countermeasures in Guinea Pigs
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    Chapter 24 Evaluation of Medical Countermeasures Against Ebolaviruses in Nonhuman Primate Models
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    Chapter 25 Quantification of Filovirus Glycoprotein-Specific Antibodies
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    Chapter 26 Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR
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    Chapter 27 Real-Time and End-Point PCR Diagnostics for Ebola Virus
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    Chapter 28 Production of Antigens for ELISA
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    Chapter 29 ELISA Methods for the Detection of Ebolavirus Infection
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    Chapter 30 Ebola Virus Field Sample Collection
Attention for Chapter 2: Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Development and Application of New Technologies
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Chapter title
Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Development and Application of New Technologies
Chapter number 2
Book title
Ebolaviruses
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7116-9_2
Pubmed ID
Book ISBNs
978-1-4939-7115-2, 978-1-4939-7116-9
Authors

Allison Groseth, Thomas Hoenen

Editors

Thomas Hoenen, Allison Groseth

Abstract

Molecular biology is a broad discipline that seeks to understand biological phenomena at a molecular level, and achieves this through the study of DNA, RNA, proteins, and/or other macromolecules (e.g., those involved in the modification of these substrates). Consequently, it relies on the availability of a wide variety of methods that deal with the collection, preservation, inactivation, separation, manipulation, imaging, and analysis of these molecules. As such the state of the art in the field of ebolavirus molecular biology research (and that of all other viruses) is largely intertwined with, if not driven by, advancements in the technical methodologies available for these kinds of studies. Here we review of the current state of our knowledge regarding ebolavirus biology and emphasize the associated methods that made these discoveries possible.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Student > Doctoral Student 2 25%
Researcher 1 13%
Unknown 3 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Medicine and Dentistry 1 13%
Unknown 3 38%