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Ebolaviruses

Overview of attention for book
Cover of 'Ebolaviruses'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Marburg- and Ebolaviruses: A Look Back and Lessons for the Future
  3. Altmetric Badge
    Chapter 2 Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Development and Application of New Technologies
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    Chapter 3 Ebolavirus: An Overview of Molecular and Clinical Pathogenesis
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    Chapter 4 Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms
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    Chapter 5 Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
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    Chapter 6 Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
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    Chapter 7 Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
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    Chapter 8 Modeling Ebolavirus Budding with Virus Like Particles
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    Chapter 9 Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays
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    Chapter 10 Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins
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    Chapter 11 Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA
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    Chapter 12 A Semi-automated High-Throughput Microtitration Assay for Filoviruses
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    Chapter 13 Generation of Recombinant Ebola Viruses Using Reverse Genetics
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    Chapter 14 Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
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    Chapter 15 Live-Cell Imaging of Filoviruses
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    Chapter 16 Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds
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    Chapter 17 Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence
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    Chapter 18 Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells
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    Chapter 19 Electron Microscopy of Ebola Virus-Infected Cells
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    Chapter 20 Validating the Inactivation Effectiveness of Chemicals on Ebola Virus
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    Chapter 21 Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)
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    Chapter 22 Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection
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    Chapter 23 Evaluation of Ebola Virus Countermeasures in Guinea Pigs
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    Chapter 24 Evaluation of Medical Countermeasures Against Ebolaviruses in Nonhuman Primate Models
  26. Altmetric Badge
    Chapter 25 Quantification of Filovirus Glycoprotein-Specific Antibodies
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    Chapter 26 Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR
  28. Altmetric Badge
    Chapter 27 Real-Time and End-Point PCR Diagnostics for Ebola Virus
  29. Altmetric Badge
    Chapter 28 Production of Antigens for ELISA
  30. Altmetric Badge
    Chapter 29 ELISA Methods for the Detection of Ebolavirus Infection
  31. Altmetric Badge
    Chapter 30 Ebola Virus Field Sample Collection
Attention for Chapter 5: Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
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Chapter title
Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
Chapter number 5
Book title
Ebolaviruses
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7116-9_5
Pubmed ID
Book ISBNs
978-1-4939-7115-2, 978-1-4939-7116-9
Authors

Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, Wendy Maury

Editors

Thomas Hoenen, Allison Groseth

Abstract

Pseudotyping lentivirus-based vectors is a strategy used to study conferred vector tropism and mechanisms of envelope glycoprotein function. Lentiviruses and filoviruses both assemble at the plasma membrane and have homotrimeric structural envelope glycoproteins that mediate both receptor binding and fusion. Such similarities help foster efficient pseudotyping. Importantly, filovirus glycoprotein pseudotyping of lentiviral vectors allows investigators to study virus entry at substantially less restrictive levels of biosafety containment than that required for wild-type filovirus work (biosafety level-2 vs. biosafety level-4, respectively). Standard lentiviral vector production involves transient transfection of viral component expression plasmids into producer cells, supernatant collection, and centrifuge concentration. Because the envelope glycoprotein expression plasmid is provided in trans, wild type or variant filoviral glycoproteins from marburgvirus or ebolavirus species may be used for pseudotyping and compared side-by-side. In this chapter we discuss the manufacture of pseudotyped lentiviral vector with an emphasis on small-scale laboratory grade production.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 31%
Student > Master 4 25%
Researcher 2 13%
Other 1 6%
Professor > Associate Professor 1 6%
Other 0 0%
Unknown 3 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 31%
Agricultural and Biological Sciences 3 19%
Immunology and Microbiology 2 13%
Medicine and Dentistry 2 13%
Physics and Astronomy 1 6%
Other 0 0%
Unknown 3 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2021.
All research outputs
#19,452,862
of 23,924,883 outputs
Outputs from Methods in molecular biology
#8,323
of 13,531 outputs
Outputs of similar age
#318,215
of 426,854 outputs
Outputs of similar age from Methods in molecular biology
#693
of 1,072 outputs
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