Report for: Ebolaviruses

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Chapter title	Production of Antigens for ELISA
Chapter number	28
Book title	Ebolaviruses
Published in	Methods in molecular biology, January 2017
DOI	10.1007/978-1-4939-7116-9_28
Pubmed ID	28573634
Book ISBNs	978-1-4939-7115-2, 978-1-4939-7116-9
Authors	Robert W. Cross, Thomas G. Ksiazek, Cross, Robert W., Ksiazek, Thomas G.
Editors	Thomas Hoenen, Allison Groseth
Abstract	Immunologic assays such as ELISA allow detection of either virus antigens or the host's immune response to antigens associated with prior infections and offer a powerful means to approach understanding the epidemiology and epizootiology of these agents. However, the success of these assays is highly dependent on the production of high-quality materials both to establish the assays (i.e., antigen target for antibody) and to serve as controls for establishing assay parameters and sensitivity. Here we describe methods for preparing ebolavirus antigens suitable for use as either reagents or controls in a variety of ELISA formats. Considering work with filoviruses is typically restricted to maximum containment laboratories; thus, we also provide methods for inactivating and safety testing of these antigens for safe use in properly established field laboratories.

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Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
United States	1	9%
Unknown	10	91%

Demographic breakdown

Readers by professional status	Count	As %
Researcher	3	27%
Other	2	18%
Student > Ph. D. Student	2	18%
Student > Master	2	18%
Student > Bachelor	1	9%
Other	0	0%
Unknown	1	9%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	4	36%
Immunology and Microbiology	2	18%
Nursing and Health Professions	1	9%
Veterinary Science and Veterinary Medicine	1	9%
Biochemistry, Genetics and Molecular Biology	1	9%
Other	1	9%
Unknown	1	9%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2022.

All research outputs

#7,591,661

of 23,146,350 outputs

Outputs from Methods in molecular biology

#2,358

of 13,275 outputs

Outputs of similar age

#141,941

of 421,846 outputs

Outputs of similar age from Methods in molecular biology

#245

of 1,075 outputs

Altmetric has tracked 23,146,350 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.

So far Altmetric has tracked 13,275 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,846 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.

We're also able to compare this research output to 1,075 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.

Ebolaviruses

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Attention Score in Context