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The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains

Overview of attention for article published in Frontiers in Microbiology, May 2017
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Title
The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains
Published in
Frontiers in Microbiology, May 2017
DOI 10.3389/fmicb.2017.00925
Pubmed ID
Authors

Ivica Dimkić, Slaviša Stanković, Marija Nišavić, Marijana Petković, Petar Ristivojević, Djordje Fira, Tanja Berić

Abstract

In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 143 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
France 1 <1%
Unknown 141 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 17%
Student > Master 24 17%
Researcher 16 11%
Student > Doctoral Student 11 8%
Student > Bachelor 11 8%
Other 18 13%
Unknown 38 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 23%
Biochemistry, Genetics and Molecular Biology 28 20%
Immunology and Microbiology 12 8%
Chemical Engineering 6 4%
Chemistry 5 3%
Other 13 9%
Unknown 46 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2017.
All research outputs
#20,427,593
of 22,979,862 outputs
Outputs from Frontiers in Microbiology
#22,632
of 25,038 outputs
Outputs of similar age
#272,964
of 313,707 outputs
Outputs of similar age from Frontiers in Microbiology
#445
of 514 outputs
Altmetric has tracked 22,979,862 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,038 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 514 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.