| Chapter title |
Expression, Purification, and Kinetic Analysis of PTP Domains
|
|---|---|
| Chapter number | 3 |
| Book title |
Protein Tyrosine Phosphatases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3746-2_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3744-8, 978-1-4939-3746-2
|
| Authors |
Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. Szedlacsek, Mentel, Mihaela, Badea, Rodica A., Necula - Petrareanu, Georgiana, Mallikarjuna, Sujay T., Ionescu, Aura E., Szedlacsek, Stefan E. |
| Abstract |
Protein tyrosine phosphatases (PTP) are a large group of enzymes which work together with protein tyrosine kinases to control the tyrosine phosphorylation of proteins, thus playing a major role in cellular signaling. Here, we provide detailed protocols for expression and purification of the catalytic domain of RPTPμ and full length Eya3 as well as the extracellular region of PTPBR7. Methods are described for evaluation of the purity of the recombinant proteins thus obtained. For the purified Eya3 phosphatase we provide protocols for enzyme activity assay using either chromogenic, fluorescent, or peptide substrates. Determination of kinetic parameters by different graphical and computer-based procedures is also described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Other | 1 | 17% |
| Lecturer > Senior Lecturer | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Unspecified | 1 | 17% |
| Environmental Science | 1 | 17% |
| Immunology and Microbiology | 1 | 17% |
| Unknown | 1 | 17% |