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Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines

Overview of attention for article published in Brazilian Journal of Medical and Biological Research, January 2016
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Title
Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
Published in
Brazilian Journal of Medical and Biological Research, January 2016
DOI 10.1590/1414-431x20165211
Pubmed ID
Authors

R.C. Teixeira, B.A. Baêta, J.S. Ferreira, R.C. Medeiros, C.M. Maya-Monteiro, F.A. Lara, L. Bell-Sakyi, A.H. Fonseca

Abstract

This study aimed to describe the association of Borrelia burgdorferi s.s. with ixodid tick cell lines by flow cytometry and fluorescence and confocal microscopy. Spirochetes were stained with a fluorescent membrane marker (PKH67 or PKH26), inoculated into 8 different tick cell lines and incubated at 30°C for 24 h. PKH efficiently stained B. burgdorferi without affecting bacterial viability or motility. Among the tick cell lines tested, the Rhipicephalus appendiculatus cell line RA243 achieved the highest percentage of association/internalization, with both high (90%) and low (10%) concentrations of BSK-H medium in tick cell culture medium. Treatment with cytochalasin D dramatically reduced the average percentage of cells with internalized spirochetes, which passed through a dramatic morphological change during their internalization by the host cell as observed in time-lapse photography. Almost all of the fluorescent bacteria were seen to be inside the tick cells. PKH labeling of borreliae proved to be a reliable and valuable tool to analyze the association of spirochetes with host cells by flow cytometry, confocal and fluorescence microscopy.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 40%
Student > Doctoral Student 3 15%
Student > Bachelor 1 5%
Other 1 5%
Student > Ph. D. Student 1 5%
Other 3 15%
Unknown 3 15%
Readers by discipline Count As %
Immunology and Microbiology 6 30%
Agricultural and Biological Sciences 3 15%
Medicine and Dentistry 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Veterinary Science and Veterinary Medicine 1 5%
Other 3 15%
Unknown 4 20%