| Chapter title |
Crystallization of Membrane Proteins: An Overview
|
|---|---|
| Chapter number | 5 |
| Book title |
Protein Crystallography
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7000-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6998-2, 978-1-4939-7000-1
|
| Authors |
Andrii Ishchenko, Enrique E. Abola, Vadim Cherezov |
| Editors |
Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski |
| Abstract |
Membrane proteins are crucial components of cellular membranes and are responsible for a variety of physiological functions. The advent of new tools and technologies for structural biology of membrane proteins has led to a significant increase in the number of structures deposited to the Protein Data Bank during the past decade. This new knowledge has expanded our fundamental understanding of their mechanism of function and contributed to the drug-design efforts. In this chapter we discuss current approaches for membrane protein expression, solubilization, crystallization, and data collection. Additionally, we describe the protein quality-control assays that are often instrumental as a guideline for a shorter path toward the structure. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 33% |
| India | 1 | 11% |
| Belgium | 1 | 11% |
| Denmark | 1 | 11% |
| France | 1 | 11% |
| Japan | 1 | 11% |
| Unknown | 1 | 11% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 56% |
| Scientists | 4 | 44% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 2% |
| Unknown | 55 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 21% |
| Student > Bachelor | 12 | 21% |
| Researcher | 11 | 20% |
| Other | 3 | 5% |
| Student > Master | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 46% |
| Agricultural and Biological Sciences | 7 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Physics and Astronomy | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 3 | 5% |
| Unknown | 14 | 25% |