Table of Contents

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   Book Overview
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   Chapter 1 Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag
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   Chapter 2 Protein Crystallization
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   Chapter 3 Advanced Methods of Protein Crystallization
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   Chapter 4 The “Sticky Patch” Model of Crystallization and Modification of Proteins for Enhanced Crystallizability
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   Chapter 5 Crystallization of Membrane Proteins: An Overview
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   Chapter 6 Locating and Visualizing Crystals for X-Ray Diffraction Experiments
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   Chapter 7 Collection of X-Ray Diffraction Data from Macromolecular Crystals
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   Chapter 8 Identifying and Overcoming Crystal Pathologies: Disorder and Twinning
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    Chapter 9 Applications of X-Ray Micro-Beam for Data Collection
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    Chapter 10 Serial Synchrotron X-Ray Crystallography (SSX)
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    Chapter 11 Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources
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    Chapter 12 Structure Determination Using X-Ray Free-Electron Laser Pulses
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    Chapter 13 Processing of XFEL Data
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    Chapter 14 Many Ways to Derivatize Macromolecules and Their Crystals for Phasing
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    Chapter 15 Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX
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    Chapter 16 Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis
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    Chapter 17 Long-Wavelength X-Ray Diffraction and Its Applications in Macromolecular Crystallography
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    Chapter 18 Acknowledging Errors: Advanced Molecular Replacement with Phaser
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    Chapter 19 Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems
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    Chapter 20 Radiation Damage in Macromolecular Crystallography
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    Chapter 21 Boxes of Model Building and Visualization
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    Chapter 22 Structure Refinement at Atomic Resolution
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    Chapter 23 Low Resolution Refinement of Atomic Models Against Crystallographic Data
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    Chapter 24 Stereochemistry and Validation of Macromolecular Structures
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    Chapter 25 Validation of Protein–Ligand Crystal Structure Models: Small Molecule and Peptide Ligands
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    Chapter 26 Protein Data Bank (PDB): The Single Global Macromolecular Structure Archive
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    Chapter 27 Databases, Repositories, and Other Data Resources in Structural Biology