Iron-Regulated Expression of Alginate Production, Mucoid Phenotype, and Biofilm Formation by Pseudomonas aeruginosa

Overview of attention for article published in mBio
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  • Above-average Attention Score compared to outputs of the same age (57th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (54th percentile)

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mendeley
58 Mendeley
Title
Iron-Regulated Expression of Alginate Production, Mucoid Phenotype, and Biofilm Formation by Pseudomonas aeruginosa
Published in
mBio
DOI 10.1128/mbio.01010-13
Pubmed ID
Authors

Jacinta R. Wiens, Adriana I. Vasil, Michael J. Schurr, Michael L. Vasil

Abstract

Pseudomonas aeruginosa strains of non-cystic fibrosis (non-CF) origin do not produce significant amounts of extracellular alginate and are nonmucoid. In CF, such isolates can become mucoid through mutation of one of the genes (mucA, mucB, mucC, or mucD) that produce regulatory factors that sequester AlgU, required for increased expression of alginate genes. Mutation of the muc genes in the nonmucoid PAO1, PA14, PAKS-1, and Ps388 strains led to increased levels of extracellular alginate and an obvious mucoid phenotype, but only under iron-limiting growth conditions (≤5 µM), not under iron-replete conditions (≥10 µM). In contrast, >50% of P. aeruginosa isolates from chronic CF pulmonary infections expressed increased levels of alginate and mucoidy both under iron-limiting and iron-replete conditions (i.e., iron-constitutive phenotype). No single iron regulatory factor (e.g., Fur, PvdS) was associated with this loss of iron-regulated alginate expression and mucoidy in these CF isolates. However, the loss of only pyoverdine production, or its uptake, abrogated the ability of P. aeruginosa to produce a robust biofilm that represents the Psl-type of biofilm. In contrast, we show that mutation of the pyoverdine and pyochelin biosynthesis genes and the pyoverdine receptor (FpvA) lead to iron-constitutive expression of the key alginate biosynthesis gene, algD, and an explicitly mucoid phenotype in both iron-limiting and iron-replete conditions. These data indicate that alginate production and mucoidy, in contrast to other types of biofilms produced by P. aeruginosa, are substantially enhanced under iron limitation. These results also have compelling implications in relation to the use of iron chelators in the treatment of P. aeruginosa CF infections.

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Belgium 1 2%
United States 1 2%
Unknown 55 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 34%
Student > Bachelor 10 17%
Student > Master 9 16%
Researcher 8 14%
Other 4 7%
Other 7 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 69%
Medicine and Dentistry 5 9%
Engineering 4 7%
Immunology and Microbiology 3 5%
Biochemistry, Genetics and Molecular Biology 2 3%
Other 4 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score was calculated when the research output was last mentioned on 06 March 2014.
All research outputs
#1,724,580
of 3,617,832 outputs
Outputs from mBio
#623
of 918 outputs
Outputs of similar age
#44,574
of 109,838 outputs
Outputs of similar age from mBio
#21
of 46 outputs
Altmetric has tracked 3,617,832 research outputs across all sources so far. This one has received more attention than most of these and is in the 50th percentile.
So far Altmetric has tracked 918 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.3. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,838 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.