Chapter title |
Rational Design of T Lymphocyte Epitope-Based Vaccines Against Coccidioides Infection
|
---|---|
Chapter number | 4 |
Book title |
Vaccines for Invasive Fungal Infections
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7104-6_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7103-9, 978-1-4939-7104-6
|
Authors |
Brady J. Hurtgen, Chiung-Yu Hung |
Editors |
Markus Kalkum, Margarita Semis |
Abstract |
Coccidioidomycosis is a potentially life-threatening mycosis endemic to the Southwestern USA and some arid regions of Central and South America. A vaccine against Coccidioides infection would benefit over 30-million people who reside in or visit the endemic regions. Vaccine candidates against systemic fungal infections come in many forms. Live attenuated vaccines are derived from disease-causing pathogens and generally stimulate excellent protective immunity. Since attenuated vaccines contain living microbes, there is a degree of unpredictability raising concerns regarding safety and stability. Generation of a subunit vaccine has initiated efforts to design a safe reagent suitable for administration to humans at risk of coccidioidomycosis. Epitope-based vaccines allow for eliciting specific protective immune responses and removal of potentially detrimental sequences to improve safety. This chapter describes methods for the identification of T cell epitopes derived from Coccidioides antigens, design, and production of a recombinant vaccine containing multiple T cell epitopes, and evaluation of its protective efficacy and vaccine immunity against pulmonary Coccidioides infection using a strain of transgenic mice that express a human MHC II molecule. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 18% |
Researcher | 2 | 18% |
Student > Ph. D. Student | 2 | 18% |
Other | 1 | 9% |
Professor | 1 | 9% |
Other | 2 | 18% |
Unknown | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 5 | 45% |
Biochemistry, Genetics and Molecular Biology | 1 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Engineering | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |