Chapter title |
Preparation of an Oral Vaccine by Proteome Analysis and Molecular Display Technology
|
---|---|
Chapter number | 16 |
Book title |
Vaccines for Invasive Fungal Infections
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7104-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7103-9, 978-1-4939-7104-6
|
Authors |
Seiji Shibasaki, Mitsuyoshi Ueda |
Editors |
Markus Kalkum, Margarita Semis |
Abstract |
In recent years, genetic engineering and protein expression technologies have promoted the development of recombinant protein vaccines. To accelerate the development of efficient vaccines for mycosis, screening candidate antigens, and determining the optimal route of administration are indispensable steps. Two methods for identifying novel antigens and producing antigens specific to Candida albicans, as a model causative pathogen of mycosis, are discussed in this chapter. Specifically, the application of liquid chromatography/tandem mass spectrometry using a long monolithic column for proteome analysis to identify virulence factors of C. albicans, followed by molecular display technology to produce an oral vaccine using antigens found by the proteomic study, is described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 2 | 33% |