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Structural basis of stereospecific reduction by quinuclidinone reductase

Overview of attention for article published in AMB Express, February 2014
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  • Among the highest-scoring outputs from this source (#49 of 199)
  • Average Attention Score compared to outputs of the same age
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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1 tweeter

Citations

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6 Dimensions

Readers on

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17 Mendeley
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Title
Structural basis of stereospecific reduction by quinuclidinone reductase
Published in
AMB Express, February 2014
DOI 10.1186/2191-0855-4-6
Pubmed ID
Abstract

Chiral molecule (R)-3-quinuclidinol, a valuable compound for the production of various pharmaceuticals, is efficiently synthesized from 3-quinuclidinone by using NADPH-dependent 3-quinuclidinone reductase (RrQR) from Rhodotorula rubra. Here, we report the crystal structure of RrQR and the structure-based mutational analysis. The enzyme forms a tetramer, in which the core of each protomer exhibits the α/β Rossmann fold and contains one molecule of NADPH, whereas the characteristic substructures of a small lobe and a variable loop are localized around the substrate-binding site. Modeling and mutation analyses of the catalytic site indicated that the hydrophobicity of two residues, I167 and F212, determines the substrate-binding orientation as well as the substrate-binding affinity. Our results revealed that the characteristic substrate-binding pocket composed of hydrophobic amino acid residues ensures substrate docking for the stereospecific reaction of RrQR in spite of its loose interaction with the substrate.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 29%
Student > Bachelor 3 18%
Professor > Associate Professor 2 12%
Student > Master 2 12%
Student > Ph. D. Student 1 6%
Other 2 12%
Unknown 2 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 29%
Chemistry 4 24%
Agricultural and Biological Sciences 4 24%
Computer Science 1 6%
Unspecified 1 6%
Other 0 0%
Unknown 2 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 February 2014.
All research outputs
#2,300,288
of 4,507,509 outputs
Outputs from AMB Express
#49
of 199 outputs
Outputs of similar age
#60,010
of 124,691 outputs
Outputs of similar age from AMB Express
#1
of 13 outputs
Altmetric has tracked 4,507,509 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 199 research outputs from this source. They receive a mean Attention Score of 1.2. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 124,691 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.