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Plant Argonaute Proteins

Overview of attention for book
Cover of 'Plant Argonaute Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
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    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
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    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
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    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
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    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
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    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
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    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
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    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
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    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
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    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
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    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
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    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
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    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
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    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
  20. Altmetric Badge
    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
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    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 6: Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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Chapter title
Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
Chapter number 6
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_6
Pubmed ID
28608336
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Carbonell, Alberto, Alberto Carbonell

Editors

Alberto Carbonell

Abstract

ARGONAUTE (AGO) proteins function in small RNA (sRNA)-based RNA silencing pathways to regulate gene expression and control invading nucleic acids. In posttranscriptional RNA silencing pathways, plant AGOs associate with sRNAs to interact with highly sequence-complementary target RNAs. Once the AGO-sRNA-target RNA ternary complex is formed, target RNA is typically repressed through AGO-mediated cleavage or through other cleavage-independent mechanisms. The universe of sRNAs associating with diverse plant AGOs has been determined though AGO immunoprecipitation (IP) and high-throughput sequencing of co-immunoprecipitated sRNAs. To better understand the biological functions of AGO-sRNA complexes, it is crucial to identify the repertoire of target RNAs they regulate. Here I present a detailed AGO-RNA IP followed by high-throughput sequencing (AGO RIP-Seq) methodology for the isolation of AGO ternary complexes from plant tissues and the high-throughput sequencing of AGO-bound target RNAs. In particular, the protocol describes the IP of slicer-deficient hemagglutinin (HA)-tagged AGO proteins expressed in plant tissues, the isolation of AGO-bound RNAs, and the generation of target RNA libraries for high-throughput sequencing.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Student > Bachelor 2 15%
Student > Master 2 15%
Unknown 5 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 46%
Agricultural and Biological Sciences 1 8%
Chemistry 1 8%
Unknown 5 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2018.
All research outputs
#13,205,576
of 22,981,247 outputs
Outputs from Methods in molecular biology
#3,431
of 13,148 outputs
Outputs of similar age
#154,718
of 317,529 outputs
Outputs of similar age from Methods in molecular biology
#52
of 301 outputs
Altmetric has tracked 22,981,247 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,148 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,529 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 301 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.

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