Report for: Plant Argonaute Proteins

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Chapter title	ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
Chapter number	12
Book title	Plant Argonaute Proteins
Published in	Methods in molecular biology, June 2017
DOI	10.1007/978-1-4939-7165-7_12
Pubmed ID	28608342
Book ISBNs	978-1-4939-7164-0, 978-1-4939-7165-7
Authors	Wang, Meizhen, Deng, Yuxing, Shao, Fenjuan, Liu, Miaomiao, Pang, Yongqi, Li, Caili, Lu, Shanfa, Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li, Shanfa Lu
Editors	Alberto Carbonell
Abstract	Small RNA-mediated gene silencing is a vital regulatory mechanism in eukaryotes that requires ARGONAUTE (AGO) proteins. Salvia miltiorrhiza is a well-known traditional Chinese medicinal plant. Therefore, it is important to characterize S. miltiorrhiza AGO family genes as they may be involved in multiple metabolic pathways. This chapter introduces the detailed protocol for SmAGO gene prediction and molecular cloning. In addition, an Agrobacterium-mediated genetic transformation method for S. miltiorrhiza is presented. These methodologies can be used to functionally study SmAGO genes as well as other genes of interest in S. miltiorrhiza.

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Plant Argonaute Proteins

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