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Plant Argonaute Proteins

Overview of attention for book
Cover of 'Plant Argonaute Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
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    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
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    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
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    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
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    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
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    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
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    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
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    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
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    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
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    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
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    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
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    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
  18. Altmetric Badge
    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
  19. Altmetric Badge
    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
  20. Altmetric Badge
    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
  21. Altmetric Badge
    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 7: Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

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Chapter title
Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
Chapter number 7
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_7
Pubmed ID
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Baksa, Ivett, Szittya, György, Ivett Baksa, György Szittya

Editors

Alberto Carbonell

Abstract

The method described here enables the high-throughput identification of cleaved mRNA targets of ARGONAUTE/small RNA complexes. The protocol is based on a modified 5'-rapid amplification of cDNA ends combined with deep sequencing of 3' cleavage products of mRNAs. Poly(A) RNA is purified from the tissue of interest which is followed by a 5'-RNA adapter ligation. The ligated products are then reverse transcribed, amplified, and digested with MmeI. After gel separation, a 3' double-stranded DNA adapter is ligated to the fragments, which are then amplified and index labeled for the high-throughput sequencing of pooled degradome libraries. Sequencing datasets from pooled libraries can be analyzed with different bioinformatic approaches.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 33%
Other 1 17%
Student > Bachelor 1 17%
Unspecified 1 17%
Researcher 1 17%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 67%
Biochemistry, Genetics and Molecular Biology 1 17%
Unspecified 1 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2018.
All research outputs
#1,720,207
of 12,695,728 outputs
Outputs from Methods in molecular biology
#431
of 8,366 outputs
Outputs of similar age
#52,189
of 265,695 outputs
Outputs of similar age from Methods in molecular biology
#3
of 19 outputs
Altmetric has tracked 12,695,728 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,366 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,695 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.