You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
|
---|---|
Chapter number | 2 |
Book title |
Plant Argonaute Proteins
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7165-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7164-0, 978-1-4939-7165-7
|
Authors |
Guodong Ren, Xiaoyan Wang, Bin Yu |
Editors |
Alberto Carbonell |
Abstract |
Uridylation (3' untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5' cleavage products (5' CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5' CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3' end uridylation of both AGO-bound miRNAs and 5' CP. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Lecturer | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Agricultural and Biological Sciences | 1 | 20% |
Medicine and Dentistry | 1 | 20% |
Unknown | 2 | 40% |