Establishment of an In Vivo ARGONAUTE Reporter System in Plants
Plant Argonaute Proteins
Methods in molecular biology, June 2017
Károly Fátyol, József Burgyán
RNA silencing is not only an evolutionarily conserved gene regulatory mechanism, but in plants also serves as the basis for robust adaptive antiviral immune responses. ARGONAUTE (AGO) proteins form the catalytic cores of the RNA-guided ribonuclease complexes, which play a central role in RNA silencing. Here we describe an in vivo assay system for analyzing the activities of AGO proteins in the virological model plant Nicotiana benthamiana .
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