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Plant Argonaute Proteins

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Cover of 'Plant Argonaute Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
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    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
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    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
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    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
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    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
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    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
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    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
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    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
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    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
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    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
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    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
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    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
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    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
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    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
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    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
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    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 13: Cloning and Characterization of Argonaute Genes in Tomato
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Chapter title
Cloning and Characterization of Argonaute Genes in Tomato
Chapter number 13
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_13
Pubmed ID
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Zhiqiang Xian, Fang Yan, Zhengguo Li

Editors

Alberto Carbonell

Abstract

Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better understand RNA silencing-based posttranscriptional regulation in fleshy fruits. Here we describe how to identify and clone SlAGO genes, as well as the methodology for their functional characterization.