Cloning and Characterization of Argonaute Genes in Tomato
Plant Argonaute Proteins
Methods in molecular biology, June 2017
Zhiqiang Xian, Fang Yan, Zhengguo Li
Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better understand RNA silencing-based posttranscriptional regulation in fleshy fruits. Here we describe how to identify and clone SlAGO genes, as well as the methodology for their functional characterization.
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