Chapter title |
Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
|
---|---|
Chapter number | 14 |
Book title |
Plant Argonaute Proteins
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7165-7_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7164-0, 978-1-4939-7165-7
|
Authors |
Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, Pablo A. Manavella |
Editors |
Alberto Carbonell |
Abstract |
Small RNAs have been traditionally detected and quantified using small RNA blots, a modified Northern blot technique. The small RNAs are size-fractionated from the rest of the cellular RNA molecules by polyacrylamide gel electrophoresis and transferred by blotting onto a positively charged membrane. A radiolabeled probe was then traditionally used to detect a specific small RNA in the cellular pool. Small RNA blotting is a relatively simple, inexpensive approach to visualize small RNAs without artifacts. However, the radioactive labeling of the probe is sometimes an impediment, especially due to the requirement of specialized facilities. Here we describe a sensitive and simple method to detect and quantify small RNAs using digoxigenin-based nonradioactive RNA blots. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Student > Master | 3 | 21% |
Researcher | 2 | 14% |
Student > Bachelor | 2 | 14% |
Professor | 1 | 7% |
Other | 2 | 14% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 36% |
Agricultural and Biological Sciences | 4 | 29% |
Medicine and Dentistry | 2 | 14% |
Immunology and Microbiology | 1 | 7% |
Unspecified | 1 | 7% |
Other | 0 | 0% |
Unknown | 1 | 7% |