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Plant Argonaute Proteins

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Cover of 'Plant Argonaute Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
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    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
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    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
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    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
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    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
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    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
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    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
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    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
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    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
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    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
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    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
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    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
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    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
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    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
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    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
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    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 18: Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
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Chapter title
Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
Chapter number 18
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_18
Pubmed ID
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Andrzej Zielezinski, Wojciech M. Karlowski

Editors

Alberto Carbonell

Abstract

WG/GW domains recruit ARGONAUTE (AGO) proteins to distinct silencing effector complexes using combinations of just two amino acids: tryptophan (W) and glycine (G), forming a wide arsenal of highly simplified interaction surfaces. These unstructured domains exhibit very low sequence identity and excessive length polymorphism, which makes identification of new AGO-binding proteins a challenging task as they escape detection with standard sequence comparison-based methods (e.g., BLAST, HMMER).In this chapter, we explain the use of tools for prediction of AGO-binding WG/GW domains in protein sequences. We also show how to computationally explore an up-to-date information about AGO-interacting proteins and discover new properties of WG/GW domains. Finally, we encourage readers to explore the game-like web application for in silico designing/modifying AGO-binding sequences as well as modeling mutagenesis experiments and predicting their potential effect on AGO-binding activity.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Researcher 1 33%
Unknown 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Unknown 1 33%