Chapter title |
Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
|
---|---|
Chapter number | 18 |
Book title |
Plant Argonaute Proteins
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7165-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7164-0, 978-1-4939-7165-7
|
Authors |
Andrzej Zielezinski, Wojciech M. Karlowski, Zielezinski, Andrzej, Karlowski, Wojciech M. |
Editors |
Alberto Carbonell |
Abstract |
WG/GW domains recruit ARGONAUTE (AGO) proteins to distinct silencing effector complexes using combinations of just two amino acids: tryptophan (W) and glycine (G), forming a wide arsenal of highly simplified interaction surfaces. These unstructured domains exhibit very low sequence identity and excessive length polymorphism, which makes identification of new AGO-binding proteins a challenging task as they escape detection with standard sequence comparison-based methods (e.g., BLAST, HMMER).In this chapter, we explain the use of tools for prediction of AGO-binding WG/GW domains in protein sequences. We also show how to computationally explore an up-to-date information about AGO-interacting proteins and discover new properties of WG/GW domains. Finally, we encourage readers to explore the game-like web application for in silico designing/modifying AGO-binding sequences as well as modeling mutagenesis experiments and predicting their potential effect on AGO-binding activity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Researcher | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 50% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 25% |
Unknown | 1 | 25% |