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Independent optical excitation of distinct neural populations

Overview of attention for article published in Nature Methods, February 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#40 of 3,934)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

news
6 news outlets
blogs
4 blogs
twitter
243 tweeters
patent
14 patents
facebook
1 Facebook page
wikipedia
2 Wikipedia pages
video
1 video uploader

Citations

dimensions_citation
777 Dimensions

Readers on

mendeley
1717 Mendeley
citeulike
2 CiteULike
Title
Independent optical excitation of distinct neural populations
Published in
Nature Methods, February 2014
DOI 10.1038/nmeth.2836
Pubmed ID
Authors

Nathan C Klapoetke, Yasunobu Murata, Sung Soo Kim, Stefan R Pulver, Amanda Birdsey-Benson, Yong Ku Cho, Tania K Morimoto, Amy S Chuong, Eric J Carpenter, Zhijian Tian, Jun Wang, Yinlong Xie, Zhixiang Yan, Yong Zhang, Brian Y Chow, Barbara Surek, Michael Melkonian, Vivek Jayaraman, Martha Constantine-Paton, Gane Ka-Shu Wong, Edward S Boyden

Abstract

Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system-mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.

Twitter Demographics

The data shown below were collected from the profiles of 243 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 1,717 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 45 3%
Germany 16 <1%
United Kingdom 11 <1%
Japan 9 <1%
Portugal 5 <1%
China 4 <1%
Netherlands 4 <1%
Austria 3 <1%
Belgium 2 <1%
Other 14 <1%
Unknown 1604 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 493 29%
Researcher 403 23%
Student > Bachelor 182 11%
Student > Master 174 10%
Student > Doctoral Student 95 6%
Other 250 15%
Unknown 120 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 638 37%
Neuroscience 482 28%
Engineering 116 7%
Biochemistry, Genetics and Molecular Biology 113 7%
Medicine and Dentistry 74 4%
Other 152 9%
Unknown 142 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 226. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2020.
All research outputs
#61,520
of 14,126,005 outputs
Outputs from Nature Methods
#40
of 3,934 outputs
Outputs of similar age
#977
of 250,992 outputs
Outputs of similar age from Nature Methods
#1
of 94 outputs
Altmetric has tracked 14,126,005 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,934 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.2. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,992 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.