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Directed Evolution Library Creation

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Cover of 'Directed Evolution Library Creation'

Table of Contents

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    Book Overview
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    Chapter 1 Directed Evolution Library Creation
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    Chapter 2 Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis
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    Chapter 3 Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli.
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    Chapter 4 The Sequence Saturation Mutagenesis (SeSaM) Method
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    Chapter 5 Generation of Effective Libraries by Neutral Drift
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    Chapter 6 Site-Saturation Mutagenesis by Overlap Extension PCR
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    Chapter 7 Iterative saturation mutagenesis: a powerful approach to engineer proteins by systematically simulating darwinian evolution.
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    Chapter 8 Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR)
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    Chapter 9 OmniChange: Simultaneous Site Saturation of Up to Five Codons
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    Chapter 10 Random Insertional–Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations
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    Chapter 11 Transposon-based approaches for generating novel molecular diversity during directed evolution.
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    Chapter 12 Restriction Enzyme-Mediated DNA Family Shuffling
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    Chapter 13 Assembly of Designed Oligonucleotides: A Useful Tool in Synthetic Biology for Creating High-Quality Combinatorial DNA Libraries
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    Chapter 14 One-Pot Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR)
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    Chapter 15 USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology.
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    Chapter 16 ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes
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    Chapter 17 Generating Random Circular Permutation Libraries
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    Chapter 18 Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate, and Diversity
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    Chapter 19 The Mutagenesis Assistant Program
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    Chapter 20 Computational Tools for Designing Smart Libraries
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    Chapter 21 Computational tools for directed evolution: a comparison of prospective and retrospective strategies.
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    Chapter 22 Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP
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    Chapter 23 Noncontiguous SCHEMA Protein Recombination.
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    Chapter 24 Engineering Proteins by Reconstructing Evolutionary Adaptive Paths
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