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A high-throughput colorimetric assay for detection of Schistosoma mansoni viability based on the tetrazolium salt XTT

Overview of attention for article published in Parasites & Vectors, June 2017
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Title
A high-throughput colorimetric assay for detection of Schistosoma mansoni viability based on the tetrazolium salt XTT
Published in
Parasites & Vectors, June 2017
DOI 10.1186/s13071-017-2240-3
Pubmed ID
Authors

Pedro Henrique Nascimento Aguiar, Núbia Monteiro Gonçalves Soares Fernandes, Carlos Leomar Zani, Marina Moraes Mourão

Abstract

Schistosoma mansoni is a trematode parasite that causes schistosomiasis, one of the most prevalent neglected tropical diseases, leading to the loss of 2.6 million disability-adjusted life years. Praziquantel is the only drug available, and new drugs are required. The most common strategy in schistosomiasis drug discovery is the use of the schistosomula larval-stage for a pre-screen in drug sensitivity assays. However, assessing schistosomula viability by microscopy has always been a limitation to the throughput of such assays. Hence, the development of validated, robust high-throughput in vitro assays for Schistosoma with simple readouts is needed. Here, we present a simple and affordable alternative to assess schistosomula viability. The method employed is based on the hydrosoluble tetrazolium salt XTT which has been widely used in other organisms but has never been used to drug screen in schistosomes. We showed that schistosomula reduce XTT salt to a coloured formazan product and that absorbance levels reflected the viability and parasites number. This XTT viability assay was validated for high throughput screening of compounds in schistosomula, and dose-response curves of compounds could be reproduced. We conclude that the XTT viability assay could be applied for the screening of large compounds collections in S. mansoni and accelerate the identification of novel antischistosomal compounds.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 23%
Researcher 5 19%
Student > Bachelor 4 15%
Other 3 12%
Student > Doctoral Student 2 8%
Other 4 15%
Unknown 2 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 35%
Agricultural and Biological Sciences 8 31%
Chemistry 2 8%
Social Sciences 1 4%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 4 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2017.
All research outputs
#9,125,160
of 11,400,239 outputs
Outputs from Parasites & Vectors
#2,096
of 2,842 outputs
Outputs of similar age
#190,301
of 263,059 outputs
Outputs of similar age from Parasites & Vectors
#125
of 136 outputs
Altmetric has tracked 11,400,239 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,842 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
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We're also able to compare this research output to 136 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.