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3D Cell Culture

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Cover of '3D Cell Culture'

Table of Contents

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    Book Overview
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    Chapter 1 3D Cell Culture: An Introduction
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    Chapter 2 Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
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    Chapter 3 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
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    Chapter 4 Hydrogel-Based In Vitro Models of Tumor Angiogenesis
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    Chapter 5 Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional Hydrogels
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    Chapter 6 Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions
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    Chapter 7 Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
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    Chapter 8 3D Coculture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial–Stromal Interactions During Mammary Branching Morphogenesis
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    Chapter 9 An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
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    Chapter 10 3D Primary Culture Model to Study Human Mammary Development
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    Chapter 11 Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
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    Chapter 12 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
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    Chapter 13 3D-Dynamic Culture Models of Multiple Myeloma
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    Chapter 14 Preparation of a Three-Dimensional Full Thickness Skin Equivalent
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    Chapter 15 Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
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    Chapter 16 3D Coculture Model of the Brain Parenchyma–Metastasis Interface of Brain Metastasis
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    Chapter 17 3D Neural Culture in Dual Hydrogel Systems
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    Chapter 18 3D Cell Culture in Micropatterned Hydrogels Prepared by Photomask, Microneedle, or Soft Lithography Techniques
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    Chapter 19 3D Stem Cell Niche Engineering via Two-Photon Laser Polymerization
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    Chapter 20 Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion
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    Chapter 21 Forecasting smog-related health hazard based on social media and physical sensor
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    Chapter 22 High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform
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    Chapter 23 High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells
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    Chapter 24 3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels
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    Chapter 25 Human Lung Small Airway-on-a-Chip Protocol
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    Chapter 26 Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs
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    Chapter 27 Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink
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    Chapter 28 Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel
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    Chapter 29 Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma
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    Chapter 30 Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
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    Chapter 31 Quantitative Phenotypic Image Analysis of Three-Dimensional Organotypic Cultures
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    Chapter 32 Erratum to: Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional Hydrogels
Attention for Chapter 27: Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink
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Chapter title
Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink
Chapter number 27
Book title
3D Cell Culture
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7021-6_27
Pubmed ID
Book ISBNs
978-1-4939-7019-3, 978-1-4939-7021-6
Authors

Pati, Falguni, Cho, Dong-Woo, Falguni Pati, Dong-Woo Cho

Editors

Zuzana Koledova

Abstract

Bioprinting provides an exciting opportunity to print and pattern all the components that make up a tissue-cells and extracellular matrix (ECM) material-in three dimensions (3D) to generate tissue analogues. A large number of materials have been used for making bioinks; however, majority of them cannot represent the complexity of natural ECM and thus are unable to reconstitute the intrinsic cellular morphologies and functions. We present here a method for making of bioink from decellularized extracellular matrices (dECMs) and a protocol for bioprinting of cell-laden constructs with this novel bioink. The dECM bioink is capable of providing an optimized microenvironment that is conducive to the growth of 3D structured tissue. We have prepared bioinks from different tissues, including adipose, cartilage and heart tissues and achieved high cell viability and functionality of the bioprinted tissue structures using our novel bioink.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 113 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 113 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 16%
Student > Bachelor 15 13%
Student > Master 11 10%
Researcher 10 9%
Student > Doctoral Student 6 5%
Other 18 16%
Unknown 35 31%
Readers by discipline Count As %
Engineering 21 19%
Medicine and Dentistry 13 12%
Biochemistry, Genetics and Molecular Biology 10 9%
Agricultural and Biological Sciences 9 8%
Materials Science 4 4%
Other 13 12%
Unknown 43 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2017.
All research outputs
#20,429,992
of 22,982,639 outputs
Outputs from Methods in molecular biology
#9,929
of 13,149 outputs
Outputs of similar age
#275,935
of 316,843 outputs
Outputs of similar age from Methods in molecular biology
#218
of 280 outputs
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