| Chapter title |
Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
|
|---|---|
| Chapter number | 7 |
| Book title |
3D Cell Culture
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7021-6_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7019-3, 978-1-4939-7021-6
|
| Authors |
Shinya Sugimoto, Toshiro Sato M.D., Ph.D., Toshiro Sato |
| Editors |
Zuzana Koledova |
| Abstract |
The intestinal epithelium is the most rapidly renewed tissue in adult mammals, and its renewal is strictly controlled by intestinal stem cells. Extensive studies using genetic models of intestinal epithelium have revealed the mechanisms underlying the self-renewal of intestinal stem cells. Exploiting this knowledge, we developed a novel 3D culture system that enables the outgrowth of intestinal Lgr5(+) stem cells derived from mouse and human tissues into ever-expanding crypt-villus mini-guts, known as intestinal epithelial organoids. These organoids are maintained by the self-renewal of stem cells and give rise to all differentiated cell types of the intestinal epithelium. Once established, organoids can be cryopreserved and thawed when needed. This culture system has been widely used for studying stem cell behavior and gene function and for disease modeling. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 86 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 20% |
| Student > Bachelor | 14 | 16% |
| Researcher | 12 | 14% |
| Student > Master | 11 | 13% |
| Student > Doctoral Student | 5 | 6% |
| Other | 4 | 5% |
| Unknown | 23 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 29 | 34% |
| Immunology and Microbiology | 11 | 13% |
| Agricultural and Biological Sciences | 8 | 9% |
| Engineering | 4 | 5% |
| Medicine and Dentistry | 3 | 3% |
| Other | 2 | 2% |
| Unknown | 29 | 34% |