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Minimal Effects of Macromolecular Crowding on an Intrinsically Disordered Protein: A Small-Angle Neutron Scattering Study

Overview of attention for article published in Biophysical Journal, February 2014
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2 tweeters

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43 Dimensions

Readers on

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63 Mendeley
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Title
Minimal Effects of Macromolecular Crowding on an Intrinsically Disordered Protein: A Small-Angle Neutron Scattering Study
Published in
Biophysical Journal, February 2014
DOI 10.1016/j.bpj.2013.12.003
Pubmed ID
Authors

David P. Goldenberg, Brian Argyle

Abstract

Small-angle neutron scattering was used to study the effects of macromolecular crowding by two globular proteins, i.e., bovine pancreatic trypsin inhibitor and equine metmyoglobin, on the conformational ensemble of an intrinsically disordered protein, the N protein of bacteriophage λ. The λ N protein was uniformly labeled with (2)H, and the concentrations of D2O in the samples were adjusted to match the neutron scattering contrast of the unlabeled crowding proteins, thereby masking their contribution to the scattering profiles. Scattering from the deuterated λ N was recorded for samples containing up to 0.12 g/mL bovine pancreatic trypsin inhibitor or 0.2 g/mL metmyoglobin. The radius of gyration of the uncrowded protein was estimated to be 30 Å and was found to be remarkably insensitive to the presence of crowders, varying by <2 Å for the highest crowder concentrations. The scattering profiles were also used to estimate the fractal dimension of λ N, which was found to be ∼1.8 in the absence or presence of crowders, indicative of a well-solvated and expanded random coil under all of the conditions examined. These results are contrary to the predictions of theoretical treatments and previous experimental studies demonstrating compaction of unfolded proteins by crowding with polymers such as dextran and Ficoll. A computational simulation suggests that some previous treatments may have overestimated the effective volumes of disordered proteins and the variation of these volumes within an ensemble. The apparent insensitivity of λ N to crowding may also be due in part to weak attractive interactions with the crowding proteins, which may compensate for the effects of steric exclusion.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 2 3%
Spain 1 2%
Italy 1 2%
Japan 1 2%
United States 1 2%
Unknown 57 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 27%
Researcher 13 21%
Student > Master 8 13%
Professor 6 10%
Student > Doctoral Student 5 8%
Other 10 16%
Unknown 4 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 35%
Physics and Astronomy 12 19%
Chemistry 8 13%
Biochemistry, Genetics and Molecular Biology 7 11%
Engineering 3 5%
Other 4 6%
Unknown 7 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2016.
All research outputs
#5,373,401
of 7,446,125 outputs
Outputs from Biophysical Journal
#2,025
of 2,820 outputs
Outputs of similar age
#103,872
of 174,645 outputs
Outputs of similar age from Biophysical Journal
#38
of 63 outputs
Altmetric has tracked 7,446,125 research outputs across all sources so far. This one is in the 24th percentile – i.e., 24% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,820 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 25th percentile – i.e., 25% of its peers scored the same or lower than it.
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We're also able to compare this research output to 63 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.