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The delayed addition of human mesenchymal stem cells to pre-formed endothelial cell networks results in functional vascularization of a collagen–glycosaminoglycan scaffold in vivo

Overview of attention for article published in Acta Biomaterialia, December 2013
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Mentioned by

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2 tweeters

Citations

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89 Dimensions

Readers on

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136 Mendeley
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Title
The delayed addition of human mesenchymal stem cells to pre-formed endothelial cell networks results in functional vascularization of a collagen–glycosaminoglycan scaffold in vivo
Published in
Acta Biomaterialia, December 2013
DOI 10.1016/j.actbio.2013.08.014
Pubmed ID
Authors

T.M. McFadden, G.P. Duffy, A.B. Allen, H.Y. Stevens, S.M. Schwarzmaier, N. Plesnila, J.M. Murphy, F.P. Barry, R.E. Guldberg, F.J. O’Brien

Abstract

This paper demonstrates a method to engineer, in vitro, a nascent microvasculature within a collagen-glycosaminoglycan scaffold with a view to overcoming the major issue of graft failure due to avascular necrosis of tissue-engineered constructs. Human umbilical vein endothelial cells (ECs) were cultured alone and in various co-culture combinations with human mesenchymal stem cells (MSCs) to determine their vasculogenic abilities in vitro. Results demonstrated that the delayed addition of MSCs to pre-formed EC networks, whereby MSCs act as pericytes to the nascent vessels, resulted in the best developed vasculature. The results also demonstrate that the crosstalk between ECs and MSCs during microvessel formation occurs in a highly regulated, spatio-temporal fashion, whereby the initial seeding of ECs results in platelet derived growth factor (PDGF) release; the subsequent addition of MSCs 3 days later leads to a cessation in PDGF production, coinciding with increased vascular endothelial cell growth factor expression and enhanced vessel formation. Functional assessment of these pre-engineered constructs in a subcutaneous rat implant model demonstrated anastomosis between the in vitro engineered vessels and the host vasculature, with significantly increased vascularization occurring in the co-culture group. This study has thus provided new information on the process of in vitro vasculogenesis within a three-dimensional porous scaffold for tissue engineering and demonstrates the potential for using these vascularized scaffolds in the repair of critical sized bone defects.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 136 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 1%
Germany 1 <1%
Brazil 1 <1%
Singapore 1 <1%
Iran, Islamic Republic of 1 <1%
United States 1 <1%
Unknown 129 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 47 35%
Researcher 21 15%
Student > Master 18 13%
Student > Bachelor 11 8%
Student > Doctoral Student 7 5%
Other 16 12%
Unknown 16 12%
Readers by discipline Count As %
Engineering 33 24%
Agricultural and Biological Sciences 33 24%
Medicine and Dentistry 14 10%
Materials Science 13 10%
Biochemistry, Genetics and Molecular Biology 6 4%
Other 15 11%
Unknown 22 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2014.
All research outputs
#7,170,565
of 12,070,659 outputs
Outputs from Acta Biomaterialia
#1,496
of 2,199 outputs
Outputs of similar age
#91,474
of 193,903 outputs
Outputs of similar age from Acta Biomaterialia
#27
of 40 outputs
Altmetric has tracked 12,070,659 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,199 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 193,903 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.