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Schrodinger’s scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer

Overview of attention for article published in BMC Genetics, February 2016
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Title
Schrodinger’s scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer
Published in
BMC Genetics, February 2016
DOI 10.1186/s12863-016-0344-y
Pubmed ID
Authors

Pranay Amruth Maroju, Sonu Yadav, Vishnupriya Kolipakam, Shweta Singh, Qamar Qureshi, Yadvendradev Jhala

Abstract

Non-invasive sampling has opened avenues for the genetic study of elusive species, which has contributed significantly to their conservation. Where field based identity of non-invasive sample is ambiguous (e.g. carnivore scats), it is essential to establish identity of the species through molecular approaches. A cost effective procedure to ascertain species identity is to use species specific primers (SSP) for PCR amplification and subsequent resolution through agarose gel electrophoresis. However, SSPs if ill designed can often cross amplify non-target sympatric species. Herein we report the problem of cross amplification with currently published SSPs, which have been used in several recent scientific articles on tigers (Panthera tigris) and leopards (Panthera pardus) in India. Since these papers form pioneering research on which future work will be based, an early rectification is required so as to not propagate this error further. We conclusively show cross amplification of three of the four SSPs, in sympatric non-target species like tiger SSP amplifying leopard and striped hyena (Hyaena hyaena), and leopard SSP amplifying tiger, lion (Panthera leo persica) and clouded leopard (Neofelis nebulosa), with the same product size. We develop and test a non-cross-amplifying leopard specific primer pair within the mitochondrial cytochrome b region. We also standardize a duplex PCR method to screen tiger and leopard samples simultaneously in one PCR reaction to reduce cost and time. These findings suggest the importance of an often overlooked preliminary protocol of conclusive identification of species from non-invasive samples. The cross amplification of published primers in conspecifics suggests the need to revisit inferences drawn by earlier work.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Sri Lanka 1 2%
Unknown 49 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 24%
Student > Ph. D. Student 8 16%
Student > Master 5 10%
Student > Bachelor 5 10%
Student > Doctoral Student 5 10%
Other 8 16%
Unknown 8 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 47%
Environmental Science 9 18%
Biochemistry, Genetics and Molecular Biology 5 10%
Computer Science 3 6%
Veterinary Science and Veterinary Medicine 2 4%
Other 1 2%
Unknown 7 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2017.
All research outputs
#12,795,099
of 16,112,117 outputs
Outputs from BMC Genetics
#686
of 1,003 outputs
Outputs of similar age
#198,054
of 270,687 outputs
Outputs of similar age from BMC Genetics
#1
of 1 outputs
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