| Chapter title |
Estrogen Receptors
|
|---|---|
| Chapter number | 2 |
| Book title |
Estrogen Receptors
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3127-9_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
| Authors |
Thekkumkara, Thomas, Snyder, Russell, Karamyan, Vardan T, Thomas Thekkumkara, Russell Snyder, Vardan T. Karamyan, Karamyan, Vardan T. |
| Abstract |
The role of 2-methoxyestradiol is becoming a major area of investigation because of its therapeutic utility, though its mechanism is not fully explored. Recent studies have identified the G-protein-coupled receptor 30 (GPR30, GPER) as a high-affinity membrane receptor for 2-methoxyestradiol. However, studies aimed at establishing the binding affinities of steroid compounds for specific targets are difficult, as the tracers are highly lipophilic and often result in nonspecific binding in lipid-rich membrane preparations with low-level target receptor expression. 2-Methoxyestradiol binding studies are essential to elucidate the underlying effects of this novel estrogen metabolite and to validate its targets; therefore, this competitive receptor-binding assay protocol was developed in order to assess the membrane receptor binding and affinity of 2-methyoxyestradiol. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 25% |
| Student > Ph. D. Student | 1 | 13% |
| Unspecified | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Student > Postgraduate | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Unspecified | 1 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Materials Science | 1 | 13% |
| Unknown | 3 | 38% |