Chapter title |
Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture
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Chapter number | 25 |
Book title |
Estrogen Receptors
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3127-9_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Natalija Eigeliene, Risto Erkkola, Pirkko Härkönen, Eigeliene, Natalija, Erkkola, Risto, Härkönen, Pirkko |
Abstract |
Explant tissue culture provides a model for studying the direct effects of steroid hormones, their analogs, and novel hormonally active compounds on normal freshly isolated human breast tissues (HBTs). For this purpose, pre- and postmenopausal HBTs can be maintained in this culture system. The results demonstrate that the morphological integrity of HBT explants can be maintained in tissue culture up to 2 weeks and expression of differentiation markers, steroid hormone receptors, proliferation and apoptosis ratios can be evaluated as a response to hormonal stimulation. This chapter describes an ex vivo culture model that we have applied to study the effects of various hormonally active substances, including 17β-estradiol and selective estrogen receptor modulators (SERMs), on normal human breast tissues. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 50% |
Student > Bachelor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Agricultural and Biological Sciences | 2 | 25% |
Medicine and Dentistry | 1 | 13% |
Engineering | 1 | 13% |
Unknown | 1 | 13% |