Chapter title |
Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromatography and Tandem Mass Spectrometry
|
---|---|
Chapter number | 11 |
Book title |
Estrogen Receptors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3127-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Eduardo A. Callegari, Callegari, Eduardo A. |
Abstract |
Shotgun (gel-free) proteomics is a useful approach to perform identification and relative quantification of protein in complex mixtures such as tissue homogenates, biological fluids, cell lysates, and extracellular proteins. Incorporation of separative and analytical techniques such as two-dimensional liquid chromatography at nanoscale (2D-nanoLC) coupled to tandem mass spectrometry (MS/MS analysis) into the shotgun protocol provides an excellent strategy. This chapter describes the application of the shotgun proteomics protocol to evaluate the identity and expression analysis of proteins from rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 25% |
Student > Bachelor | 3 | 25% |
Unspecified | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 25% |
Engineering | 2 | 17% |
Unspecified | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Computer Science | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 33% |