Chapter title |
FRET-Based Real-Time DNA Microarrays
|
---|---|
Chapter number | 12 |
Book title |
Functional Genomics
|
Published in |
Methods in molecular biology, November 2011
|
DOI | 10.1007/978-1-61779-424-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-61779-423-0, 978-1-61779-424-7
|
Authors |
Babak Hassibi, Arjang Hassibi, Haris Vikalo, José Luis Riechmann |
Editors |
Michael Kaufmann, Claudia Klinger |
Abstract |
We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e., real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation, washing artifacts, microarray spot-to-spot variations, and other intensity-affecting impediments. We demonstrate in both theory and practice that the time-constant of target capturing is inversely proportional to the concentration of the target analyte, which we take advantage of as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to experimentally validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays. |
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Biochemistry, Genetics and Molecular Biology | 1 | 50% |
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