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Functional Genomics

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Cover of 'Functional Genomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Prediction of Protein Tertiary Structures Using MUFOLD.
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    Chapter 2 Prediction of protein functions.
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    Chapter 3 Genome-wide screens for expressed hypothetical proteins.
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    Chapter 4 Self-Custom-Made SFP Arrays for Nonmodel Organisms.
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    Chapter 5 Construction and analysis of full-length and normalized cDNA libraries from citrus.
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    Chapter 6 Assembling linear DNA templates for in vitro transcription and translation.
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    Chapter 7 Automated Computational Analysis of Genome-Wide DNA Methylation Profiling Data from HELP-Tagging Assays.
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    Chapter 8 Detection of RNA Editing Events in Human Cells Using High-Throughput Sequencing
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    Chapter 9 Comparative study of differential gene expression in closely related bacterial species by comparative hybridization.
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    Chapter 10 Whole-Genome RT-qPCR MicroRNA Expression Profiling
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    Chapter 11 Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to Validate Gene Regulation by PTTG.
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    Chapter 12 FRET-Based Real-Time DNA Microarrays
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    Chapter 13 2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps.
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    Chapter 14 The use of antigen microarrays in antibody profiling.
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    Chapter 15 Limited proteolysis in proteomics using protease-immobilized microreactors.
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    Chapter 16 Mass spectrometry for protein quantification in biomarker discovery.
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    Chapter 17 High-throughput microtitre plate-based assay for DNA topoisomerases.
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    Chapter 18 Microscale Thermophoresis as a Sensitive Method to Quantify Protein: Nucleic Acid Interactions in Solution
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    Chapter 19 Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.
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    Chapter 20 LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System.
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    Chapter 21 Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins.
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    Chapter 22 Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene.
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    Chapter 23 Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli.
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    Chapter 24 Functional screen of zebrafish deubiquitylating enzymes by morpholino knockdown and in situ hybridization.
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    Chapter 25 Silencing of gene expression by gymnotic delivery of antisense oligonucleotides.
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    Chapter 26 Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters.
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    Chapter 27 Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy.
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    Chapter 28 Metabolome Analysis of Gram-Positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS.
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    Chapter 29 Metabolic Fingerprinting Using Comprehensive Two-Dimensional Gas Chromatography - Time-of-Flight Mass Spectrometry.
Attention for Chapter 12: FRET-Based Real-Time DNA Microarrays
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Chapter title
FRET-Based Real-Time DNA Microarrays
Chapter number 12
Book title
Functional Genomics
Published in
Methods in molecular biology, November 2011
DOI 10.1007/978-1-61779-424-7_12
Pubmed ID
Book ISBNs
978-1-61779-423-0, 978-1-61779-424-7
Authors

Babak Hassibi, Arjang Hassibi, Haris Vikalo, José Luis Riechmann

Editors

Michael Kaufmann, Claudia Klinger

Abstract

We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e., real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation, washing artifacts, microarray spot-to-spot variations, and other intensity-affecting impediments. We demonstrate in both theory and practice that the time-constant of target capturing is inversely proportional to the concentration of the target analyte, which we take advantage of as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to experimentally validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Unknown 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2013.
All research outputs
#18,327,422
of 22,694,633 outputs
Outputs from Methods in molecular biology
#7,835
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Outputs of similar age
#116,377
of 141,655 outputs
Outputs of similar age from Methods in molecular biology
#35
of 76 outputs
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