Functional Genomics

Table of Contents

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   Book Overview
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   Chapter 1 Prediction of Protein Tertiary Structures Using MUFOLD.
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   Chapter 2 Prediction of protein functions.
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   Chapter 3 Genome-wide screens for expressed hypothetical proteins.
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   Chapter 4 Self-Custom-Made SFP Arrays for Nonmodel Organisms.
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   Chapter 5 Construction and analysis of full-length and normalized cDNA libraries from citrus.
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   Chapter 6 Assembling linear DNA templates for in vitro transcription and translation.
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   Chapter 7 Automated Computational Analysis of Genome-Wide DNA Methylation Profiling Data from HELP-Tagging Assays.
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   Chapter 8 Detection of RNA Editing Events in Human Cells Using High-Throughput Sequencing
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    Chapter 9 Comparative study of differential gene expression in closely related bacterial species by comparative hybridization.
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    Chapter 10 Whole-Genome RT-qPCR MicroRNA Expression Profiling
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    Chapter 11 Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to Validate Gene Regulation by PTTG.
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    Chapter 12 FRET-Based Real-Time DNA Microarrays
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    Chapter 13 2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps.
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    Chapter 14 The use of antigen microarrays in antibody profiling.
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    Chapter 15 Limited proteolysis in proteomics using protease-immobilized microreactors.
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    Chapter 16 Mass spectrometry for protein quantification in biomarker discovery.
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    Chapter 17 High-throughput microtitre plate-based assay for DNA topoisomerases.
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    Chapter 18 Microscale Thermophoresis as a Sensitive Method to Quantify Protein: Nucleic Acid Interactions in Solution
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    Chapter 19 Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.
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    Chapter 20 LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System.
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    Chapter 21 Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins.
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    Chapter 22 Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene.
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    Chapter 23 Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli.
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    Chapter 24 Functional screen of zebrafish deubiquitylating enzymes by morpholino knockdown and in situ hybridization.
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    Chapter 25 Silencing of gene expression by gymnotic delivery of antisense oligonucleotides.
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    Chapter 26 Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters.
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    Chapter 27 Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy.
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    Chapter 28 Metabolome Analysis of Gram-Positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS.
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    Chapter 29 Metabolic Fingerprinting Using Comprehensive Two-Dimensional Gas Chromatography - Time-of-Flight Mass Spectrometry.