| Chapter title |
PCR ITS-RFLP for Penicillium Species and Other Genera
|
|---|---|
| Chapter number | 21 |
| Book title |
Mycotoxigenic Fungi
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6707-0_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
| Authors |
Sandrine Rousseaux, Michèle Guilloux-Bénatier, Rousseaux, Sandrine, Guilloux-Bénatier, Michèle |
| Abstract |
Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and among them 41 different species. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 20% |
| Professor > Associate Professor | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Lecturer > Senior Lecturer | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Agricultural and Biological Sciences | 3 | 30% |
| Unknown | 4 | 40% |