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Mycotoxigenic Fungi

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Cover of 'Mycotoxigenic Fungi'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Mycotoxins: An Underhand Food Problem
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    Chapter 2 Alternaria Species and Their Associated Mycotoxins
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    Chapter 3 Aspergillus Species and Their Associated Mycotoxins
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    Chapter 4 Fusarium Species and Their Associated Mycotoxins
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    Chapter 5 Penicillium Species and Their Associated Mycotoxins
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    Chapter 6 Targeting Conserved Genes in Alternaria Species
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    Chapter 7 Targeting Conserved Genes in Aspergillus Species
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    Chapter 8 Targeting Conserved Genes in Fusarium Species
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    Chapter 9 Targeting Conserved Genes in Penicillium Species
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    Chapter 10 Targeting Aflatoxin Biosynthetic Genes
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    Chapter 11 Targeting Trichothecene Biosynthetic Genes
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    Chapter 12 Targeting Ochratoxin Biosynthetic Genes
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    Chapter 13 Targeting Fumonisin Biosynthetic Genes
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    Chapter 14 Targeting Other Mycotoxin Biosynthetic Genes
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    Chapter 15 Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
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    Chapter 16 Evaluating Fumonisin Gene Expression in Fusarium verticillioides
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    Chapter 17 Multiplex Detection of Aspergillus Species
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    Chapter 18 Multiplex Detection of Fusarium Species
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    Chapter 19 Multiplex Detection of Toxigenic Penicillium Species
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    Chapter 20 PCR-RFLP for Aspergillus Species
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    Chapter 21 PCR ITS-RFLP for Penicillium Species and Other Genera
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    Chapter 22 Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
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    Chapter 23 Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
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    Chapter 24 Mycotoxins: A Fungal Genomics Perspective
Attention for Chapter 20: PCR-RFLP for Aspergillus Species
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Chapter title
PCR-RFLP for Aspergillus Species
Chapter number 20
Book title
Mycotoxigenic Fungi
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6707-0_20
Pubmed ID
Book ISBNs
978-1-4939-6705-6, 978-1-4939-6707-0
Authors

Ali Atoui, André El Khoury

Abstract

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is the most simple method for single-nucleotide change detection. It is widely used in the detection and differentiation between mycotoxigenic species. It is based on PCR amplification of a target region containing the variant site of the studied species followed by restriction endonuclease digestion and gel electrophoresis to visualize the RFLP patterns. In this method primers are designed to flank the polymorphic site and positioned in such a way as to create unequally sized fragments upon restriction endonuclease cleavage of the PCR products. Here, we describe the protocol of PCR-RFLP developed for the detection and differentiation between Aspergillus flavus and A. parasiticus by amplifying a 674 bp fragment of the aflR-aflJ intergenic region followed by restriction endonuclease analysis using BglII to obtain RFLP patterns.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 22%
Student > Ph. D. Student 1 11%
Professor 1 11%
Lecturer 1 11%
Researcher 1 11%
Other 0 0%
Unknown 3 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 22%
Immunology and Microbiology 1 11%
Social Sciences 1 11%
Medicine and Dentistry 1 11%
Unknown 4 44%