Title |
The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS
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Published in |
Marine Drugs, June 2017
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DOI | 10.3390/md15070201 |
Pubmed ID | |
Authors |
Flaviana Di Lorenzo, Angelo Palmigiano, Ida Paciello, Mateusz Pallach, Domenico Garozzo, Maria-Lina Bernardini, Violetta La Cono, Michail M. Yakimov, Antonio Molinaro, Alba Silipo |
Abstract |
The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroideslacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS. |
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Geographical breakdown
Country | Count | As % |
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Italy | 2 | 29% |
Switzerland | 2 | 29% |
Unknown | 3 | 43% |
Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 2 | 29% |
Scientists | 1 | 14% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Ph. D. Student | 8 | 35% |
Researcher | 4 | 17% |
Student > Doctoral Student | 1 | 4% |
Student > Bachelor | 1 | 4% |
Professor | 1 | 4% |
Other | 3 | 13% |
Unknown | 5 | 22% |
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Social Sciences | 1 | 4% |
Unknown | 7 | 30% |